Flow cytometry is a powerful technique used to quantitively measure individual cells and other particles in suspension, at the rate of thousands of cells per second. Most commonly used in the context of immunology, we have also started to see the adaption of flow cytometry for environmental studies, extracellular vesicle analysis, and the ability to measure many parameters simultaneously, in both conventional and spectral formats, for more comprehensive analysis. Agilent flow cytometers offer excellent capabilities, high quality data, and an easy to use platform to save researchers time when acquiring and analyzing data.