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應(yīng)用的篩選條件
本應(yīng)用簡報(bào)為使用 XF 線粒體毒性檢測(cè)進(jìn)行化合物篩選和劑量響應(yīng)實(shí)驗(yàn)以及使用線粒 體毒性指標(biāo) (MTI) 解析結(jié)果數(shù)據(jù)提供了概念驗(yàn)證和示例。
| 英語 (美國) | 完整版（PDF） |
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概述安捷倫 Seahorse XF 細(xì)胞線粒體壓力測(cè)試和XF PMP工作流程如何應(yīng)用于線粒體和代謝靶點(diǎn)的識(shí)別研究。
Overview of the benefits of the Agilent Seahorse XF tissue metabolism workflow using the Seahorse XF Flex Analyzer and the Seahorse XF Flex 3D Capture Microplates-L
An optimized Agilent XF 3D tissue workflow that allows for detection and quantitative assessment of energy metabolism in tissue materials is presented.
Demonstration of the wide range of metabolic rates that can be quantified on the Agilent Seahorse XF Flex analyzer through the use of different Seahorse XF microplates and in vitro differentiation of adipocytes.
Operating manual for the Agilent Seahorse XF Flex instrument.
Research highlight confirming the critical role of metabolic fitness in CAR-NK cells and its importance to achieve superior clinical outcomes in immunotherapy.
This document is an information guide and checklist prepared for you that outlines the supplies, space, and utility requirements for the system set up in your lab.
This application note introduces a workflow that uses the XF Real-Time ATP rate assay data for identifying metabolic modulator and drug targets.
Characterize metabolic functionality of adipocytes differentiated from the pre-adipocyte 3Y3-L1 cell culture line using the Agilent Seahorse XF/XFe Analyzer.
Protocol to assess primary whole islet bioenergetics in vitro using the Agilent Seahorse XF24 Islet Capture Microplate.
Application Brief- summarizes scientific Agilent-Seahorse XF research focused on neurodegeneration, immunology, and infectious disease.
Find out more about this advanced technique for conducting an XFe Assay at low Oxygen levels.
Supplemental procedures to perform XF assays in hypoxia chambers using the Agilent Seahorse XF Analyzer.
Mitochondrial function correlates to brown adipocyte function
Measuring white adipose tissue (WAT) mitochondrial function reveals impaired fatty acid stimulated uncoupling from obese individuals.
Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment
Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer
Prepare adherent monolayer cultures of biological samples normally grown in suspension, such as lymphocytes, for assay on the Agilent Seahorse XFe/XF24 Analyzers.
Prepare adherent monolayer cultures of biological samples normally grown in suspension, such as lymphocytes, for assay on the Agilent Seahorse XFe/XF96 Analyzers.