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Charge variant analysis of monoclonal antibodies with pH gradients using the Agilent 1260 Infinity Bio-inert Quaternary LC with alternating column regeneration.
ASMS 2023 Poster - The present study describes a novel native 2D-SEC-MS-WCX-MS workflow that enables us to characterize and quantify aggregates and charge variants of mAb A through native mass spectrometry.