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CAR T-cell manufacturing conditions are critical to differentiation, metabolic fitness, and cytotoxic function, enhancing therapy efficacy and production efficiency.
Describes use of a tethering approach to immobilize liquid tumor cell lines on to the surface of gold biosensors embedded in an Agilent E-Plate.
Poster presented at AAI 2025. Immune cell-mediated cytotoxicity of CD19 CAR-T cells against Raji suspension tumor cells was monitored and measured using real-time whole-well live cellimaging on the xCELLigence RTCA eSight system with its 5x...
This application note demonstrates a robust method for performing real-time antibody internalization assays using the Agilent xCELLigence RTCA eSight combined with CypHer5E pH-sensitive dyes.
In this study, we present a robust approach to monitor and analyze the bystander effect of Antibody-Drug Conjugates in real-time using live-cell imaging andimpedance measurements with the Agilent xCELLigence RTCA eSight system.
AACR 2025 poster: Chronic anti-CD3/CD28 stimulation during cell expansion generates exhausted T cells with a distinct metabolic and cytotoxic phenotype
Discover Agilent's automated imaging-based method for robust analysis of CAR T cell cytotoxicity, enhancing cancer treatment efficacy and research efficiency.
There is a need for functional potency assays that can be used to rapidly test the efficacy of different immunotherapy approaches, including but not limited tosmall molecules, biologics, cell therapies, or combinations of these agents
The eSight can be used to study cytotoxicity kinetics of lymphocytes invading a deregulated extracellular matrix present in solid tumors.
we demonstrated consistent production of CAR T-cells using the functionally closed and automated cell manufacturing Lonza Cocoon platform, using a novelcombination of cell analysis parameters obtained from an Agilent xCELLigence RTCA system, a...