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Targeting the immune system has dramatically increased the need for tracking the native, and more recently, engineered phenotypes of multiple cell lineages.
The Agilent NovoCyte Flow Cytometer has become the backbone of the Department of Clinical Immunology lab at Aarhus University Hospital due its ease-of-use and stability.
In this application note, cytokine production was measured for 27 different cytokines using the BioLegend LEGENDplex COVID-19 cytokine storm panels 1 and 2.
Describes the advancements in detector technology, and highlights examples where optimal signal resolution is essential for acquiring quality data.
A 24-color immunophenotyping panel for the five-laser Agilent NovoCyte Penteon flow cytometer was designed for human peripheral blood to characterize the major immune cell subsets, providing a comprehensive overview of immune cell status.
NovoCyte Penteon Flow Cytometer Operator's Guide (RUO)
To efficiently determine optimal CAR construction, researchers must develop a robust screening protocol to identify the evaluate cells and cytokytic potency. Here, we describe a workflow using a combination of the Agilent xCELLigence RTCA analyzer...
Using a sentinel panel design, a 16-color panel using 28 different antibodies can be designed for the three-laser Agilent NovoCyte Advanteon flow cytometer.
A tumor cell spiking assay was performed to determine the sensitivity and accuracy of rare cell detection on the Agilent NovoCyte Quanteon flow cytometer.
Generational proliferation was monitored with the NovoCyte Quanteon in peripheral blood mononuclear cells (PBMCs) using cell tracking dyes, with automated flow cytometric analysis with Agilent NovoExpress software.
Examines the phosphorylation of signal transducer and activator of transcription (STAT) proteins in response to cytokine stimulation using a phosphoflow technique.
A description of an 18-color immunophenotyping stain being used to examine leukocyte subsets in human peripheral blood mononuclear cells (PBMCs).
Describes the ability of the Agilent NovoCyte Quanteon flow cytometer to resolve small particles, including cell-derived extracellular vesicles (EV) and beads.